Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.
Identifieur interne : 000200 ( Main/Exploration ); précédent : 000199; suivant : 000201Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.
Auteurs : Frederico Bruno-Moreno [Brésil] ; Raquel Sombra Basílio De Oliveira ; Renato De Azevedo Moreira ; Marina Duarte Pinto Lobo ; Cléverson Diniz Teixeira De Freitas ; Márcio Viana Ramos ; Thalles Barbosa Grangeiro ; Ana Cristina Oliveira Monteiro-MoreiraSource :
- Acta crystallographica. Section F, Structural biology and crystallization communications [ 1744-3091 ] ; 2013.
Descripteurs français
- KwdFr :
- Antifongiques (composition chimique), Antifongiques (isolement et purification), Calotropis (MeSH), Cristallisation (MeSH), Diffraction des rayons X (MeSH), Extraits de plantes (composition chimique), Extraits de plantes (isolement et purification), Latex (composition chimique), Latex (isolement et purification), Protéines végétales (composition chimique), Protéines végétales (isolement et purification), Spectrométrie de masse ESI (MeSH).
- MESH :
- composition chimique : Antifongiques, Extraits de plantes, Latex, Protéines végétales.
- isolement et purification : Antifongiques, Extraits de plantes, Latex, Protéines végétales.
- Calotropis, Cristallisation, Diffraction des rayons X, Spectrométrie de masse ESI.
English descriptors
- KwdEn :
- Antifungal Agents (chemistry), Antifungal Agents (isolation & purification), Calotropis (MeSH), Crystallization (MeSH), Latex (chemistry), Latex (isolation & purification), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Proteins (chemistry), Plant Proteins (isolation & purification), Spectrometry, Mass, Electrospray Ionization (MeSH), X-Ray Diffraction (MeSH).
- MESH :
- chemical , chemistry : Antifungal Agents, Latex, Plant Extracts, Plant Proteins.
- chemical , isolation & purification : Antifungal Agents, Latex, Plant Extracts, Plant Proteins.
- Calotropis, Crystallization, Spectrometry, Mass, Electrospray Ionization, X-Ray Diffraction.
Abstract
An osmotin (CpOsm) from the latex of Calotropis procera has been crystallized in both tetragonal and trigonal forms suitable for structure determination. Crystallographic studies of CpOsm are of great interest because limited information is available concerning the structure of latex proteins and CpOsm has previously been shown to interact with the spore membranes of some plant pathogenic fungi, thus impairing spore germination and hyphal growth. CpOsm crystals were grown using 0.1 M HEPES buffer pH 7.5, 26% PEG 4000, 0.2 M ammonium sulfate (space group P4(3)) or using 0.1 M HEPES buffer pH 7.5, 35% MPD, 0.7 M ammonium sulfate (space group P3(1)12). X-ray diffraction data were collected to 2.17 Å (P4(3)) and 1.80 Å (P3(1)12) resolution and molecular-replacement analyses produced initial phases for both crystal forms.
DOI: 10.1107/S1744309113011378
PubMed: 23722843
PubMed Central: PMC3668584
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.</title><author><name sortKey="Bruno Moreno, Frederico" sort="Bruno Moreno, Frederico" uniqKey="Bruno Moreno F" first="Frederico" last="Bruno-Moreno">Frederico Bruno-Moreno</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Avenida Washington Soares 1321, Bairro Edson Queiroz, 60811-905 Fortaleza-CE, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Ciências da Saúde, Universidade de Fortaleza, Avenida Washington Soares 1321, Bairro Edson Queiroz, 60811-905 Fortaleza-CE</wicri:regionArea><placeName><region type="state">Ceará</region></placeName></affiliation></author><author><name sortKey="Sombra Basilio De Oliveira, Raquel" sort="Sombra Basilio De Oliveira, Raquel" uniqKey="Sombra Basilio De Oliveira R" first="Raquel" last="Sombra Basílio De Oliveira">Raquel Sombra Basílio De Oliveira</name></author><author><name sortKey="De Azevedo Moreira, Renato" sort="De Azevedo Moreira, Renato" uniqKey="De Azevedo Moreira R" first="Renato" last="De Azevedo Moreira">Renato De Azevedo Moreira</name></author><author><name sortKey="Pinto Lobo, Marina Duarte" sort="Pinto Lobo, Marina Duarte" uniqKey="Pinto Lobo M" first="Marina Duarte" last="Pinto Lobo">Marina Duarte Pinto Lobo</name></author><author><name sortKey="Teixeira De Freitas, Cleverson Diniz" sort="Teixeira De Freitas, Cleverson Diniz" uniqKey="Teixeira De Freitas C" first="Cléverson Diniz" last="Teixeira De Freitas">Cléverson Diniz Teixeira De Freitas</name></author><author><name sortKey="Viana Ramos, Marcio" sort="Viana Ramos, Marcio" uniqKey="Viana Ramos M" first="Márcio" last="Viana Ramos">Márcio Viana Ramos</name></author><author><name sortKey="Barbosa Grangeiro, Thalles" sort="Barbosa Grangeiro, Thalles" uniqKey="Barbosa Grangeiro T" first="Thalles" last="Barbosa Grangeiro">Thalles Barbosa Grangeiro</name></author><author><name sortKey="Oliveira Monteiro Moreira, Ana Cristina" sort="Oliveira Monteiro Moreira, Ana Cristina" uniqKey="Oliveira Monteiro Moreira A" first="Ana Cristina" last="Oliveira Monteiro-Moreira">Ana Cristina Oliveira Monteiro-Moreira</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23722843</idno><idno type="pmid">23722843</idno><idno type="doi">10.1107/S1744309113011378</idno><idno type="pmc">PMC3668584</idno><idno type="wicri:Area/Main/Corpus">000191</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000191</idno><idno type="wicri:Area/Main/Curation">000191</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000191</idno><idno type="wicri:Area/Main/Exploration">000191</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.</title><author><name sortKey="Bruno Moreno, Frederico" sort="Bruno Moreno, Frederico" uniqKey="Bruno Moreno F" first="Frederico" last="Bruno-Moreno">Frederico Bruno-Moreno</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Avenida Washington Soares 1321, Bairro Edson Queiroz, 60811-905 Fortaleza-CE, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Ciências da Saúde, Universidade de Fortaleza, Avenida Washington Soares 1321, Bairro Edson Queiroz, 60811-905 Fortaleza-CE</wicri:regionArea><placeName><region type="state">Ceará</region></placeName></affiliation></author><author><name sortKey="Sombra Basilio De Oliveira, Raquel" sort="Sombra Basilio De Oliveira, Raquel" uniqKey="Sombra Basilio De Oliveira R" first="Raquel" last="Sombra Basílio De Oliveira">Raquel Sombra Basílio De Oliveira</name></author><author><name sortKey="De Azevedo Moreira, Renato" sort="De Azevedo Moreira, Renato" uniqKey="De Azevedo Moreira R" first="Renato" last="De Azevedo Moreira">Renato De Azevedo Moreira</name></author><author><name sortKey="Pinto Lobo, Marina Duarte" sort="Pinto Lobo, Marina Duarte" uniqKey="Pinto Lobo M" first="Marina Duarte" last="Pinto Lobo">Marina Duarte Pinto Lobo</name></author><author><name sortKey="Teixeira De Freitas, Cleverson Diniz" sort="Teixeira De Freitas, Cleverson Diniz" uniqKey="Teixeira De Freitas C" first="Cléverson Diniz" last="Teixeira De Freitas">Cléverson Diniz Teixeira De Freitas</name></author><author><name sortKey="Viana Ramos, Marcio" sort="Viana Ramos, Marcio" uniqKey="Viana Ramos M" first="Márcio" last="Viana Ramos">Márcio Viana Ramos</name></author><author><name sortKey="Barbosa Grangeiro, Thalles" sort="Barbosa Grangeiro, Thalles" uniqKey="Barbosa Grangeiro T" first="Thalles" last="Barbosa Grangeiro">Thalles Barbosa Grangeiro</name></author><author><name sortKey="Oliveira Monteiro Moreira, Ana Cristina" sort="Oliveira Monteiro Moreira, Ana Cristina" uniqKey="Oliveira Monteiro Moreira A" first="Ana Cristina" last="Oliveira Monteiro-Moreira">Ana Cristina Oliveira Monteiro-Moreira</name></author></analytic><series><title level="j">Acta crystallographica. Section F, Structural biology and crystallization communications</title><idno type="eISSN">1744-3091</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal Agents (chemistry)</term><term>Antifungal Agents (isolation & purification)</term><term>Calotropis (MeSH)</term><term>Crystallization (MeSH)</term><term>Latex (chemistry)</term><term>Latex (isolation & purification)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (isolation & purification)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (isolation & purification)</term><term>Spectrometry, Mass, Electrospray Ionization (MeSH)</term><term>X-Ray Diffraction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (composition chimique)</term><term>Antifongiques (isolement et purification)</term><term>Calotropis (MeSH)</term><term>Cristallisation (MeSH)</term><term>Diffraction des rayons X (MeSH)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (isolement et purification)</term><term>Latex (composition chimique)</term><term>Latex (isolement et purification)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (isolement et purification)</term><term>Spectrométrie de masse ESI (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term><term>Latex</term><term>Plant Extracts</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antifungal Agents</term><term>Latex</term><term>Plant Extracts</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antifongiques</term><term>Extraits de plantes</term><term>Latex</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antifongiques</term><term>Extraits de plantes</term><term>Latex</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Calotropis</term><term>Crystallization</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>X-Ray Diffraction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Calotropis</term><term>Cristallisation</term><term>Diffraction des rayons X</term><term>Spectrométrie de masse ESI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An osmotin (CpOsm) from the latex of Calotropis procera has been crystallized in both tetragonal and trigonal forms suitable for structure determination. Crystallographic studies of CpOsm are of great interest because limited information is available concerning the structure of latex proteins and CpOsm has previously been shown to interact with the spore membranes of some plant pathogenic fungi, thus impairing spore germination and hyphal growth. CpOsm crystals were grown using 0.1 M HEPES buffer pH 7.5, 26% PEG 4000, 0.2 M ammonium sulfate (space group P4(3)) or using 0.1 M HEPES buffer pH 7.5, 35% MPD, 0.7 M ammonium sulfate (space group P3(1)12). X-ray diffraction data were collected to 2.17 Å (P4(3)) and 1.80 Å (P3(1)12) resolution and molecular-replacement analyses produced initial phases for both crystal forms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23722843</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1744-3091</ISSN><JournalIssue CitedMedium="Internet"><Volume>69</Volume><Issue>Pt 6</Issue><PubDate><Year>2013</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Acta crystallographica. Section F, Structural biology and crystallization communications</Title><ISOAbbreviation>Acta Crystallogr Sect F Struct Biol Cryst Commun</ISOAbbreviation></Journal><ArticleTitle>Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.</ArticleTitle><Pagination><MedlinePgn>646-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1107/S1744309113011378</ELocationID><Abstract><AbstractText>An osmotin (CpOsm) from the latex of Calotropis procera has been crystallized in both tetragonal and trigonal forms suitable for structure determination. Crystallographic studies of CpOsm are of great interest because limited information is available concerning the structure of latex proteins and CpOsm has previously been shown to interact with the spore membranes of some plant pathogenic fungi, thus impairing spore germination and hyphal growth. CpOsm crystals were grown using 0.1 M HEPES buffer pH 7.5, 26% PEG 4000, 0.2 M ammonium sulfate (space group P4(3)) or using 0.1 M HEPES buffer pH 7.5, 35% MPD, 0.7 M ammonium sulfate (space group P3(1)12). X-ray diffraction data were collected to 2.17 Å (P4(3)) and 1.80 Å (P3(1)12) resolution and molecular-replacement analyses produced initial phases for both crystal forms.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bruno-Moreno</LastName><ForeName>Frederico</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centro de Ciências da Saúde, Universidade de Fortaleza, Avenida Washington Soares 1321, Bairro Edson Queiroz, 60811-905 Fortaleza-CE, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sombra Basílio de Oliveira</LastName><ForeName>Raquel</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>de Azevedo Moreira</LastName><ForeName>Renato</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Pinto Lobo</LastName><ForeName>Marina Duarte</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Teixeira de Freitas</LastName><ForeName>Cléverson Diniz</ForeName><Initials>CD</Initials></Author><Author ValidYN="Y"><LastName>Viana Ramos</LastName><ForeName>Márcio</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Barbosa Grangeiro</LastName><ForeName>Thalles</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Oliveira Monteiro-Moreira</LastName><ForeName>Ana Cristina</ForeName><Initials>AC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>05</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Acta Crystallogr Sect F Struct Biol Cryst Commun</MedlineTA><NlmUniqueID>101226117</NlmUniqueID><ISSNLinking>1744-3091</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007840">Latex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031107" MajorTopicYN="Y">Calotropis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="N">Crystallization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007840" MajorTopicYN="N">Latex</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014961" MajorTopicYN="N">X-Ray Diffraction</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Calotropis procera</Keyword><Keyword MajorTopicYN="N">PR-5</Keyword><Keyword MajorTopicYN="N">latex proteins</Keyword><Keyword MajorTopicYN="N">osmotins</Keyword><Keyword MajorTopicYN="N">thaumatin-like proteins</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>04</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>6</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>6</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23722843</ArticleId><ArticleId IdType="pii">S1744309113011378</ArticleId><ArticleId IdType="doi">10.1107/S1744309113011378</ArticleId><ArticleId IdType="pmc">PMC3668584</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1987 Oct;85(2):529-36</Citation><ArticleIdList><ArticleId IdType="pubmed">16665731</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1991 Jun;3(6):619-28</Citation><ArticleIdList><ArticleId IdType="pubmed">1841721</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2011 Dec 23;286(51):43726-34</Citation><ArticleIdList><ArticleId IdType="pubmed">22027836</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytochemistry. 2009 May;70(8):970-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19527911</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2006;44:135-62</Citation><ArticleIdList><ArticleId IdType="pubmed">16602946</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1968 Apr 28;33(2):491-7</Citation><ArticleIdList><ArticleId IdType="pubmed">5700707</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2011 Jul;234(1):183-93</Citation><ArticleIdList><ArticleId IdType="pubmed">21394468</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42</Citation><ArticleIdList><ArticleId IdType="pubmed">21460441</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2006 Oct;44(10):574-80</Citation><ArticleIdList><ArticleId IdType="pubmed">17070691</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2011 Jul;49(7):738-43</Citation><ArticleIdList><ArticleId IdType="pubmed">21334906</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytochemistry. 2011 Sep;72(13):1510-30</Citation><ArticleIdList><ArticleId IdType="pubmed">21450319</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2008 Dec;13(12):631-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18977166</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteins. 2004 Jan 1;54(1):170-3</Citation><ArticleIdList><ArticleId IdType="pubmed">14705035</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2011 Oct;1808(10):2501-7</Citation><ArticleIdList><ArticleId IdType="pubmed">21798235</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>Ceará</li></region></list><tree><noCountry><name sortKey="Barbosa Grangeiro, Thalles" sort="Barbosa Grangeiro, Thalles" uniqKey="Barbosa Grangeiro T" first="Thalles" last="Barbosa Grangeiro">Thalles Barbosa Grangeiro</name><name sortKey="De Azevedo Moreira, Renato" sort="De Azevedo Moreira, Renato" uniqKey="De Azevedo Moreira R" first="Renato" last="De Azevedo Moreira">Renato De Azevedo Moreira</name><name sortKey="Oliveira Monteiro Moreira, Ana Cristina" sort="Oliveira Monteiro Moreira, Ana Cristina" uniqKey="Oliveira Monteiro Moreira A" first="Ana Cristina" last="Oliveira Monteiro-Moreira">Ana Cristina Oliveira Monteiro-Moreira</name><name sortKey="Pinto Lobo, Marina Duarte" sort="Pinto Lobo, Marina Duarte" uniqKey="Pinto Lobo M" first="Marina Duarte" last="Pinto Lobo">Marina Duarte Pinto Lobo</name><name sortKey="Sombra Basilio De Oliveira, Raquel" sort="Sombra Basilio De Oliveira, Raquel" uniqKey="Sombra Basilio De Oliveira R" first="Raquel" last="Sombra Basílio De Oliveira">Raquel Sombra Basílio De Oliveira</name><name sortKey="Teixeira De Freitas, Cleverson Diniz" sort="Teixeira De Freitas, Cleverson Diniz" uniqKey="Teixeira De Freitas C" first="Cléverson Diniz" last="Teixeira De Freitas">Cléverson Diniz Teixeira De Freitas</name><name sortKey="Viana Ramos, Marcio" sort="Viana Ramos, Marcio" uniqKey="Viana Ramos M" first="Márcio" last="Viana Ramos">Márcio Viana Ramos</name></noCountry><country name="Brésil"><region name="Ceará"><name sortKey="Bruno Moreno, Frederico" sort="Bruno Moreno, Frederico" uniqKey="Bruno Moreno F" first="Frederico" last="Bruno-Moreno">Frederico Bruno-Moreno</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/ThaumatinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000200 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000200 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= ThaumatinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23722843
|texte= Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23722843" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ThaumatinV1
| This area was generated with Dilib version V0.6.37. |  |